Cold fusion cloning kit manual

Cold Fusion Cloning Kit Cat. #s MCA-1, MCA-1 (Toll Free) (outside US) Page 7 E. Transformation 1. Add 50μl Cold Fusion competent cells to the cloning mixture 2. Incubate on ice for 20 minutes 3. Heat shock at 42˚C for 50 seconds 4. Transfer on ice for 2 minutes 5. Add μl S.O.C medium or LB broth 6.  · The Cold Fusion technology is a simple, rapid and highly efficient cloning kit. It allows you to directly clone any PCR product(s) to any linearized expression vector, at any site. The PCR fragments can be generated by Taq DNA polymerase or other high fidelity DNA polymerases, with primers that are designed to have at least 15 bases of homology at their linear ends to where you want the product to Estimated Reading Time: 3 mins. ClonExpress II One Step Cloning (Vazyme, #C) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at .

In-Fusion® HD Cloning Kit User Manual () www.doorway.ru Takara Bio USA, Inc. Page 5 of 15 II. List of Components All In-Fusion HD Cloning kits contain 5X In-Fusion HD Enzyme Premix, linearized pUC19 Control Vector (50 ng/μl), and 2 kb Control Insert (40 ng/μl). Store all components at –20°C. In-Fusion™ Advantage PCR Cloning Kit User Manual Protocol No. PT www.doorway.ru Clontech Laboratories, Inc. Version No. PR9Z A Takara Bio Company 4 II. In-Fusion Advantage Protocol Overview The table below is a general outline of the protocol used in the In-Fusion Advantage PCR Cloning Kits. This outline is further illustrated in. ClonExpress II One Step Cloning (Vazyme, #C) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site.

Seamless Cloning with In-Fusion PCR Cloning Kits. Save time, be efficient and don't Compromise on Flexibility. Experimental workflow of single fragment insertion into a vector using the GeneCopoeia Fast-FusionTM Cloning Kit. Page 3. Fast-FusionTM Cloning Kit User Manual. Dear Xiaoying Liang, I am planing to use In-Fusion cloning kit to cloned 4 fragments together (<1kb each) within a pcDNA(+) vector (~5 kb). Which protocol.